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Hello,
Given that each tool used in the pipeline has multiple options/flags, I was wondering if there is a place where I can specify certain parameters other than the few lines in the config.ini file.
Im running into an issue of my reads not getting merged
Vsearch Merge pairs
vsearch v2.7.1_linux_x86_64, 187.5GB RAM, 16 cores
https://github.com/torognes/vsearch
Merging reads 100%
63708703 Pairs
24311 Merged (0.0%)
63684392 Not merged (100.0%)
Pairs that failed merging due to various reasons:
14256882 too few kmers found on same diagonal
57798 potential tandem repeat
89 too many differences
49121432 alignment score too low, or score drop to high
248191 staggered read pairs
and I would like to try some changes in the parameters of VSEARCH. Please advise. Thanks!
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