Get UW RNASeq quote

[sr320]

120 extracted fish tissue samples for RNA seq.
72 brain samples- many with low RIN scores - 48 will be gill RNA

[kubu4]

Sorry, what's the total sample count?

[sr320]

120

[kubu4]

And, just to confirm, these will be submitted at total RNA, correct (i.e. they will require polyA selection or rRNA removal)?

[kubu4]

Quote requested.

[sr320]

Yes- that is correct.

…

On Mon, Dec 22, 2025 at 2:18 PM kubu4 ***@***.***> wrote: *kubu4* left a comment (RobertsLab/resources#2397) <https://urldefense.com/v3/__https://github.com/RobertsLab/resources/issues/2397*issuecomment-3684378230__;Iw!!K-Hz7m0Vt54!golwqu5xWh5kgBy5BUaVxKNZ0WgxPP0qRmpn7Cr7OLUt0t62JemgXtACBqZ52oVyKS78Tyz1YDBeYoFLI03zZfI$> And, just to confirm, these will be submitted at total RNA, correct (i.e. they will require polyA selection or rRNA removal)? — Reply to this email directly, view it on GitHub <https://urldefense.com/v3/__https://github.com/RobertsLab/resources/issues/2397*issuecomment-3684378230__;Iw!!K-Hz7m0Vt54!golwqu5xWh5kgBy5BUaVxKNZ0WgxPP0qRmpn7Cr7OLUt0t62JemgXtACBqZ52oVyKS78Tyz1YDBeYoFLI03zZfI$>, or unsubscribe <https://urldefense.com/v3/__https://github.com/notifications/unsubscribe-auth/ABB4PN6IHDME4TB3TL3LTSL4DBU2XAVCNFSM6AAAAACPZKVWRKVHI2DSMVQWIX3LMV43OSLTON2WKQ3PNVWWK3TUHMZTMOBUGM3TQMRTGA__;!!K-Hz7m0Vt54!golwqu5xWh5kgBy5BUaVxKNZ0WgxPP0qRmpn7Cr7OLUt0t62JemgXtACBqZ52oVyKS78Tyz1YDBeYoFLRFyzWew$> . You are receiving this because you authored the thread.Message ID: ***@***.***>

[sr320]

QC data

_NGS_Dittman_samples RNA quality.xlsx
Sample QC report of_30-1047869163_250404044133.pdf
SampleQC_1-48_30-1047869163.pdf
SampleQC-1-27,29-31,49-72-30-1047869163.pdf
SampleQC-28,32-48-30-1047869163.pdf

[kubu4]

Quote: $25,713.85

Quote-2025-12_025.pdf

[sr320]

@kubu4 Lets proceed with this - at UW. What is the next step - note we already have QC data #2397 (comment)

And there is an experiment that might be subject to different prep strategies. - Not sure if they will go use these data or prefer to do their own QC.

[kubu4]

What would be an estimated drop-off date?

[sr320]

Would estimate next week. Samples are at NOAA Montlake.

[kubu4]

Cool.

And, are we good with them proceeding with a "best effort", considering RIN scores are low for many samples?

[sr320]

All queries and future coordination with core we would prefer to have go directly to our collaborator -). Note there is a particular concern on 72 brain samples - separate recommendation was subject those to ribosomal reduction. Collaborator would like core's opinion out that.

[kubu4]

So, I'll let Andy takeover from here, then?

[sr320]

Yes please

…

On Wed, Jan 28, 2026 at 11:55 AM kubu4 ***@***.***> wrote: *kubu4* left a comment (RobertsLab/resources#2397) <https://urldefense.com/v3/__https://github.com/RobertsLab/resources/issues/2397*issuecomment-3811767046__;Iw!!K-Hz7m0Vt54!i9wpR3v8MnwGzDQXcUmLEkmMkaORuB6QYoJMtbs_fzr6qRNrn0D6TH1ykWVSlKR03vspxDK83lTDHaZk9Q8Uop4$> So, I'll let Andy takeover from here, then? — Reply to this email directly, view it on GitHub <https://urldefense.com/v3/__https://github.com/RobertsLab/resources/issues/2397*issuecomment-3811767046__;Iw!!K-Hz7m0Vt54!i9wpR3v8MnwGzDQXcUmLEkmMkaORuB6QYoJMtbs_fzr6qRNrn0D6TH1ykWVSlKR03vspxDK83lTDHaZk9Q8Uop4$>, or unsubscribe <https://urldefense.com/v3/__https://github.com/notifications/unsubscribe-auth/ABB4PN2UGXOJFHPXUORAKGL4JDEXJAVCNFSM6AAAAACPZKVWRKVHI2DSMVQWIX3LMV43OSLTON2WKQ3PNVWWK3TUHMZTQMJRG43DOMBUGY__;!!K-Hz7m0Vt54!i9wpR3v8MnwGzDQXcUmLEkmMkaORuB6QYoJMtbs_fzr6qRNrn0D6TH1ykWVSlKR03vspxDK83lTDHaZkI_UJZ6k$> . You are receiving this because you authored the thread.Message ID: ***@***.***>