Generating reads with even 10X coverage for WGS is taking too long

[tnnandi]

Hi,

I'm trying to generate reads with 10X coverage for a genome with a size of 3 billion bp and a read length of 2x150 bp. This leads to a requirement of 60 million reads and it is turning out to be almost impossible to generate all the reads using a single CPU. Is there a way to accelerate this reads generation process?

Thank you very much.

[davetang]

Split your genome up into chromosomes (or contigs), run dwgsim on each separately, and then concatenate the FASTQ (and mutation) files. If you didn't already know, you can directly cat the gz files.