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This software will always be open source, and available to any researcher using it for non-profit work. For the moment, the software is designed around intensity-based optoretinography, or iORG processing. That means that it is capable of processing/analyzing data from any en-face device that produces videos, such as scanning laser ophthalmoscopes or line-scan ophthalmoscopes, and their adaptive optics variants (e.g. AO-SLO/AO-LSO/AO-FiO).
This wiki in particular is designed to help with the creation of configuration files, as well as provide explanations to the variety of configuration options available to a user. If you're just looking to "get up and go", see the Quick Start page. Otherwise, keep reading, and see how deep the rabbit hole goes...
Currently, 𝑓(Cell) performs its analyses in the following stages; only the required stages are shown.
flowchart LR
subgraph preanalysis["Pre-Analysis Pipeline"]
direction TB
AA(Load Dataset) --> BB([Parse Tags/Metadata])
BB([Parse Tags/Metadata]) --> HH([Intra-Video Torsion Removal])
HH([Intra-Video Torsion Removal]) --> II(Data Output and Sorting)
end
preanalysis --> analysis
subgraph analysis["Analysis"]
direction TB
A(Load Dataset) --> B([Parse Tags/Metadata])
B([Parse Tags/Metadata]) --> D([Segment Query Points / Extract ORGs])
D([Segment Query Points / Extract ORG]) --> E([Standardize ORGs])
E([Standardize ORGs]) --> F([Summarize ORGs])
end
click BB "https://github.com/OCVL/F-Cell/wiki/Advanced:-Tag-Parsing" "Tag Parsing"
click CC "https://github.com/OCVL/F-Cell/wiki/Advanced:-Pre%E2%80%90Analysis-Pipeline#general-parameters" "Custom Steps"
click DD "https://github.com/OCVL/F-Cell/wiki/Advanced:-Pre%E2%80%90Analysis-Pipeline#trimming" "Trim Video"
click EE "https://github.com/OCVL/F-Cell/wiki/Advanced:-Pre%E2%80%90Analysis-Pipeline#flat-fielding" "Flat Field"
click FF "https://github.com/OCVL/F-Cell/wiki/Advanced:-Pre%E2%80%90Analysis-Pipeline#blurring" "Blurring"
click GG "https://github.com/OCVL/F-Cell/wiki/Advanced:-Pre%E2%80%90Analysis-Pipeline#roi-masking" "Crop Video"
click HH "https://github.com/OCVL/F-Cell/wiki/Advanced:-Pre%E2%80%90Analysis-Pipeline#torsion-correction" "Intra-Video Torsion Removal"
click B "https://github.com/OCVL/F-Cell/wiki/Advanced:-Tag-Parsing" "Tag Parsing"
click C "https://github.com/OCVL/F-Cell/wiki/Advanced:-Analysis-Parameters#data-normalization" "Normalize Data"
click D "https://github.com/OCVL/F-Cell/wiki/Advanced:-Analysis-Parameters#query-point-segmentation" "Query Point Segmentation"
click E "https://github.com/OCVL/F-Cell/wiki/Advanced:-Analysis-Parameters#signal-standardization" "Standardization"
click F "https://github.com/OCVL/F-Cell/wiki/Advanced:-Analysis-Parameters#org-summary" "Summarization"
click G "https://github.com/OCVL/F-Cell/wiki/Advanced:-Analysis-Metrics" "Metrics"
click H "https://github.com/OCVL/F-Cell/wiki/Advanced:-Analysis-Display-Parameters" "Display Results"