Ageing-analysis-using-R

Bioinformatics research on ageing hypothesis based on the chromosomal sequences and specific nucleotide repeats in various organisms using sequin package in R programming for analysis.

Telomere Repeat Analysis in Multiple Organisms

🧬 This repository presents my B.Tech thesis project, where I analyzed telomeric repeat patterns (TTAGGG and variants) in the genome of 28 organisms using R.

📂 Included Files

• B.Tech Thesis.pdf: Full report including methodology, literature review, and results
• telomere_repeat_analysis.R: R script used to detect telomeric repeat positions and generate plots

🔧 Tools Used

• R programming language
• seqinr package in R
• Genomic FASTA files from NCBI

🔍 Summary

• Focused on repeats: TTAGGG, GGGATT, AATCCC, and CCCTAA
• Analyzed both forward and reverse strands
• Visualized repeat counts using histograms
• Results indicate conserved presence of repeats across diverse phyla

📊 Key Organisms

• Drosophila, C. elegans, Human, Whale, Cat, Dolphin, Honeybee, Panda, etc.

📈 Findings

• Internal telomeric repeats are conserved across species
• Differences in repeat distribution suggest evolutionary roles
• Data may help in future synthetic biology and aging research

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🧑‍🎓 Author: Jyothi Swaroop C