SEM Particle Analyser

SEM Particle Analyser (or SEM-PSD) is a desktop application for particle size distribution (PSD) analysis of scanning electron microscopy (SEM) images. It provides a reproducible, interactive, and user-friendly workflow for extracting particle statistics from high-resolution microscopy data.

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✨ Key Features

• Wide image support

  • 8-bit and 16-bit grayscale SEM images.
  • Formats: TIFF, PNG, JPEG, BMP.

• Automatic scale detection

  • Reads µm/pixel calibration from TIFF metadata.
  • Manual scale override available.

• Flexible preprocessing

  • Region of interest (ROI) cropping (exclude top, bottom, left, right).
  • Background leveling & CLAHE contrast enhancement.
  • Top-hat filtering to highlight small bright particles.

• Adaptive or Otsu thresholding

  • Fully configurable block size and offset C.

• Morphological operations

  • Opening / closing in real-world units (µm).
  • Automatic hole filling.

• Watershed segmentation

  • Split touching particles by adjustable neck size & minimum segment size.

• Interactive GUI (PySide6)

  • Preview tabs: Original, Leveled, Threshold, Overlay, Histogram, Cumulative PSD.
  • Particle statistics: Count, D10, D50, D90, mean, std, min, max.
  • Table of particle diameters.

• Interactive exclusions

  • Toggle Remove blobs mode → click on a contour to exclude (red).
  • Click again to restore.
  • Excluded particles are grey/italic in the table.
  • Statistics, plots, and CSV export consider only active (green) particles.

• Export options

  • CSV of particle diameters.
  • Overlay images with contours (PNG/JPEG).

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📦 Requirements

• Python ≥ 3.9
• PySide6
• OpenCV (cv2)
• Pillow
• NumPy
• Matplotlib

Install dependencies:

pip install PySide6 opencv-python pillow numpy matplotlib