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Shashank-Bioinfo/README.md

Hi there, I'm Shashank Kashyap! 👋

Bioinformatics | Computational Drug Discovery | Data Science

I am a Biotechnology Engineering graduate with a passion for decoding biological complexity using computational tools. My work bridges the gap between wet-lab biology and data science, focusing on Transcriptomics, Structure-based Drug Design, and Systems Biology.

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🛠️ Technical Skills

  • Languages: Python (Pandas, Scipy, NetworkX), R (Seurat, ClusterProfiler).
  • Bioinformatics: scRNA-seq Analysis, Molecular Docking (AutoDock), Network Topology.
  • Machine Learning: KNN, Classification, ROC Analysis, Feature Extraction.
  • Tools: PyMOL, Discovery Studio, SwissADME, STRING DB.

🧬 Featured Projects

Here is an overview of my key research projects, demonstrating my ability to analyze multi-omics data and develop therapeutic hypotheses.

1. 🦠 Single-Cell Transcriptomics (Oncology)

Project: Drug Response Profiling of TNBC using scRNA-seq

  • Objective: Dissected the tumor microenvironment of Triple-Negative Breast Cancer to understand resistance to chemo-immunotherapy.
  • Key Skills: R, Seurat, Differential Gene Expression, Pathway Enrichment.
  • Outcome: Identified IL1R2+ M2 Macrophages and FOXP3+ Tregs as drivers of resistance; proposed 3 novel therapeutic targets.
  • View Repository »

2. 💊 In-Silico Drug Discovery (Neuroscience)

Project: Multi-Target Drug Design for Alzheimer’s Disease

  • Objective: Screened natural compounds to inhibit AChE and BACE1 enzymes and designed a hydrogel delivery system for BBB permeability.
  • Key Skills: Molecular Docking (AutoDock), ADMET Profiling (SwissADME), Protein Validation.
  • Outcome: Identified Withanolide A (-13.90 kcal/mol) as a high-affinity dual-inhibitor.
  • View Repository »

3. 🕸️ Systems Biology & Network Analysis (Virology)

Project: Host-Virus Interaction Network Analysis (SARS-CoV-2 & Influenza)

  • Objective: Developed a Python pipeline to map conserved host dependency factors and "Bridge Proteins" connecting viral interactomes.
  • Key Skills: Python, NetworkX, Graph Theory, Statistical Validation (Hypergeometric test).
  • Outcome: Discovered a hidden PI3K/AKT signaling hub connecting both viruses; prioritized TCF12 as a broad-spectrum antiviral target.
  • View Repository »

4. 🧠 Biomedical Signal Processing (Machine Learning)

Project: Motor Imagery EEG Signal Classification

  • Objective: Built a Machine Learning pipeline to classify left vs. right-hand movements from noisy EEG signals for BCI applications.
  • Key Skills: Signal Processing (FFT), Feature Engineering (Frequency Domain), Cubic KNN.
  • Outcome: Achieved 97.6% accuracy (Class 1) using a Cubic KNN classifier with Peak Frequency features.
  • View Repository »

"Driven by data, inspired by biology."

Popular repositories Loading

  1. host-virus-network-analysis host-virus-network-analysis Public

    Broad-spectrum antiviral target discovery for SARS-CoV-2 and Influenza A.

  2. tnbc-scrna-seq-analysis tnbc-scrna-seq-analysis Public

    Single-cell transcriptomic profiling of TNBC tumor microenvironment using R (Seurat) to map immune heterogeneity and resistance drivers.

  3. alzheimers-drug-design alzheimers-drug-design Public

    Computational drug discovery pipeline targeting Alzheimer's Disease: Virtual screening, ADMET profiling, and molecular docking of natural inhibitors.

  4. eeg_signal_classification eeg_signal_classification Public

    A Machine Learning pipeline for Brain-Computer Interfaces (BCI) that classifies Motor Imagery EEG signals (Left vs. Right Hand). Utilizes Frequency Domain feature extraction and a Cubic KNN classif…

  5. Shashank-Bioinfo Shashank-Bioinfo Public

    Bioinformatics & Data Science Portfolio: Genomics | Structural Biology | Network Analysis | BCI Signal Processing.