BioToolkit

This is a small bioinformatics toolkit for working with DNA and RNA sequences be dadaist2001.

What it can do

• DNA tools: check if a sequence is DNA, transcribe DNA to RNA, reverse, complement, reverse complement.
• RNA tools: check if a sequence is RNA, reverse transcribe RNA to DNA, reverse, complement, reverse complement.
• Filter FASTQ sequences by GC content, length, or quality..
• Bioinformatics file utilities (HW5):
  • convert_multiline_fasta_to_oneline(input_fasta, output_fasta=None) – converts multi-line FASTA sequences to single-line format.
  • parse_blast_output(input_file, output_file) – extracts top hits from BLAST reports and saves them sorted.
  • select_genes_from_gbk_to_fasta – extracts neighboring genes from GenBank files into FASTA format (monster-function).

How to use it

Clone the repository:

git clone https://github.com/YOUR_USERNAME/BioToolkit.git
cd BioToolkit

For Python

from BioToolkit import run_dna_rna_tools from BioToolkit import filter_fastq from BioToolkit import convert_multiline_fasta_to_oneline from BioToolkit import parse_blast_output from BioToolkit import select_genes_from_gbk_to_fasta