How to normalise different sequencing coverage samples in CNVKit?

[tanbiswas]

Hi
I'm using CNVKit to identify copy number alterations using WES data. However, our tumor samples have a coverage of 200x and corresponding normal samples have a coverage of 100x. So, is there a way to normalize the coverage before calling CNV using CNVKit?

Please let me know hoe to proceed.

[bahreinia]

Hi,
I am having the same question. We are using a panel of normal samples with 100x coverage as the reference for each tumor sample with a coverage of 300x. I would appreciate if someone could help.
Thanks

[etal]

Yes, the sequencing depth normalization is automatic.